Journal: BMC Cancer

Article Title: IL-36 s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer?

doi: 10.1186/s12885-020-6587-z

Figure Lengend Snippet: Comparison of the production of IL-36α, IL-36β and IL-36γ between non-cancer colon tissue versus cancer tissue in CRC ( a , d and g , respectively), using Wilcoxon signed-rank test. Y-axis represents arbitrary image units. Representative images of IL-36α, IL-36β and IL-36γ production are illustrated in the microphotographs for non-cancer ( b , e , and h ) and cancer tissues ( c f and i ). ****: P < 0.0001. The bar represents 30 μm

Article Snippet: Sections (4 μm) from TAMs were checked for consistency with previous histopathological evaluation by HE staining, prior to labelled with rabbit anti-human IL-36α (1/1200) (ab180909), rabbit anti-human IL-36β (1/3200) (ab180890) and rat anti-human IL-36γ (1/1200) (ab156783) antibodies (Abcam, Cambridge, UK).

Techniques: Comparison

Journal: BMC Cancer

Article Title: IL-36 s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer?

doi: 10.1186/s12885-020-6587-z

Figure Lengend Snippet: The ROC curve and survival curve analysis of the production of IL-36α, IL-36β and IL-36γ among the CRC patients. The specificity versus sensitivity of IL-36α, IL-36β and IL-36γ ( a ) in CRC is shown using ROC curves ( a ). Area under the curve, IL-36α: AUC = 0.68; IL-36β: AUC = 0.73; IL-36γ: AUC = 0.65. Survival curves comparing high and low levels of production of IL-36α ( b ), IL-36β ( c ) and IL-36γ ( d ) are shown, using the Kaplan-Meier method and the log-rank test

Article Snippet: Sections (4 μm) from TAMs were checked for consistency with previous histopathological evaluation by HE staining, prior to labelled with rabbit anti-human IL-36α (1/1200) (ab180909), rabbit anti-human IL-36β (1/3200) (ab180890) and rat anti-human IL-36γ (1/1200) (ab156783) antibodies (Abcam, Cambridge, UK).

Techniques:

Journal: BMC Cancer

Article Title: IL-36 s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer?

doi: 10.1186/s12885-020-6587-z

Figure Lengend Snippet: Survival curve analysis for sub-groups of CRC patients when defined by combined IL-36α and IL-36β production level ( a ) or IL-36α and IL-36γ ( b ), or IL-36β and IL-36γ ( c ), using the Kaplan-Meier method and the log-rank test

Article Snippet: Sections (4 μm) from TAMs were checked for consistency with previous histopathological evaluation by HE staining, prior to labelled with rabbit anti-human IL-36α (1/1200) (ab180909), rabbit anti-human IL-36β (1/3200) (ab180890) and rat anti-human IL-36γ (1/1200) (ab156783) antibodies (Abcam, Cambridge, UK).

Techniques:

Journal: BMC Cancer

Article Title: IL-36 s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer?

doi: 10.1186/s12885-020-6587-z

Figure Lengend Snippet: Survival curve analysis for sub-groups of CRC patients when defined by clinical presentation at surgery, comparing high and low expression levels of IL-36 s: by IL-36α production in patients with an invasion depth of T4 ( a ), lymph node metastasis ( c ), TNM III-IV ( e ), or right-sided colon CRC ( g ); or by IL-36γ production in patients with an invasion depth of T1–3 ( b ), no lymph node metastasis ( d ), TNM I-II ( f ), or left-sided colon CRC ( h ), using the Kaplan-Meier method and the log-rank test. X-axis represents days elapsed

Article Snippet: Sections (4 μm) from TAMs were checked for consistency with previous histopathological evaluation by HE staining, prior to labelled with rabbit anti-human IL-36α (1/1200) (ab180909), rabbit anti-human IL-36β (1/3200) (ab180890) and rat anti-human IL-36γ (1/1200) (ab156783) antibodies (Abcam, Cambridge, UK).

Techniques: Expressing

Journal: BMC Cancer

Article Title: IL-36 s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer?

doi: 10.1186/s12885-020-6587-z

Figure Lengend Snippet: Univariate and multivariate analysis of IL-36 s and clinicopathological factors affecting survival of patients with CRC

Article Snippet: Sections (4 μm) from TAMs were checked for consistency with previous histopathological evaluation by HE staining, prior to labelled with rabbit anti-human IL-36α (1/1200) (ab180909), rabbit anti-human IL-36β (1/3200) (ab180890) and rat anti-human IL-36γ (1/1200) (ab156783) antibodies (Abcam, Cambridge, UK).

Techniques:

Journal: The Journal of investigative dermatology

Article Title: Unprocessed interleukin-36α regulates psoriasis-like skin inflammation in co-operation with interleukin-1

doi: 10.1038/jid.2015.289

Figure Lengend Snippet: Wild type, IL-36α −/− , IL-36β −/− and IL-36γ −/− mice were treated with imiquimod for 4 days. The day after the last application anesthetized ( a ) or euthanized ( b ) mice were photographed and skin collected after euthanasia. Control mice were denuded and left untreated until skin collection. Skin was examined by H&E staining ( c–d ) and immunohistochemistry for PCNA ( e–f ). Representative images from 5 (IL-36α −/− and IL-36β −/− ) and 3 (IL-36γ −/− ) independent imiquimod experiments are shown. Blue scale bars = 200 µm. Black scale bars = 50 µm.

Article Snippet: Individual IL-36 cytokines were detected by sequential incubation with: 1) goat anti-IL-36α (AF2297, R&D Systems), or goat anti-IL-36β (AF2298, R&D Systems) and 2) appropriate species-specific horseradish peroxidase conjugated secondary antibodies.

Techniques: Control, Staining, Immunohistochemistry

Journal: The Journal of investigative dermatology

Article Title: Unprocessed interleukin-36α regulates psoriasis-like skin inflammation in co-operation with interleukin-1

doi: 10.1038/jid.2015.289

Figure Lengend Snippet: Wild type ( a and b ), IL-36α −/− ( a ), IL-36β −/− ( a ) and IL-36γ −/− ( b ) mice were treated with imiquimod for 4 days as described in . Skin was collected the day after the last imiquimod application and examined by H&E staining or immunohistochemistry for neutrophils (Ly-6G/Ly-6C). Red arrows indicate Munro’s microabscesses (clusters of neutrophils) at the top of the epidermis. Black arrows indicate individual neutrophils within the dermis. Representative images from the experiments also analyzed in and are shown. Scale bars = 50 µm.

Article Snippet: Individual IL-36 cytokines were detected by sequential incubation with: 1) goat anti-IL-36α (AF2297, R&D Systems), or goat anti-IL-36β (AF2298, R&D Systems) and 2) appropriate species-specific horseradish peroxidase conjugated secondary antibodies.

Techniques: Staining, Immunohistochemistry